A solid phase radioimmunoassay for the detection of anti-insulin antibodies.
- 1 January 1983
- journal article
- research article
- Published by Japan Endocrine Society in Endocrinologia Japonica
- Vol. 30 (1) , 23-28
- https://doi.org/10.1507/endocrj1954.30.23
Abstract
A solid phase radioimmunoassay for the detection of anti-insulin antibodies was developed and evaluated. After coating round-bottomed wells of polyvinyl chloride with porcine monocomponent insulin, 50 .mu.l of serum from either insulin-treated diabetics or nondiabetic controls was added to the wells, followed by the addition of 125I-protein A. The radioactivity of the bound 125I-protein A on the wells was counted. This solid phase radioimmunoassay is very simple and does not require centrifugal steps. The results correlated well with those of previously published immune precipitation methods (r = 0.68, P < 0.01). This method requires only 50 .mu.l of serum and 4 h for the assay if the wells are coated with insulin in advance. This procedure is thought to be suitable for clinical use.This publication has 0 references indexed in Scilit: