Polyethylene glycol-facilitated transformation of Bacteroides fragilis with plasmid DNA
- 1 October 1985
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 164 (1) , 466-469
- https://doi.org/10.1128/jb.164.1.466-469.1985
Abstract
A method for the transformation of Bacteroides fragilis with plasmid DNA was developed by using the clindamycin resistance plasmid pBFTM10 as the source of transforming DNA. The method was technically simple to perform and resulted in an average of 4.2 .times. 103 transformants per .mu.g of pBFTM10 added. A method for the preparation of frozen competent cells is also described.This publication has 16 references indexed in Scilit:
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