Structural characterization and biosynthesis of gp87, a melanoma‐associated oncofetal antigen defined by monoclonal antibody 140.240

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Abstract
The monocolnal antibody 140.240, which reacts with a melanoma‐associated oncofetal antigen, identifies an epitope present on an 87 kd molecule present on the surface of melanoma cells. This molecule is a singlechain monomer, which by tunicamycin treatment and two‐dimensional tryptic mapping has been shown to arise from a 77 kd polypeptide precursor (p77). This precursor is rapidly glycosylated to an 83 kd intermediate (gp83) which in turn is rapidly further glycosylated into the mature 87 kd glycopolypeptie (gp87). Neuraminidase treatment of the glycopeptides indicates that only gp87 contains accessible sialic acid moieties.

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