EFFECT OF DIHYDROSTREPTOMYCIN ON TETRAZOLIUM DYE REDUCTION IN ESCHERICHIA COLI

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Abstract
Sonic-disrupted ex-tracts of Escherichia coli grown without added antibiotic (sensitive and resistant) contained (in supernatant of fraction centrifuged at 100,000 X g) a dihydrostreptomycin-inhabitable succinate-triphenyltetrazolium chloride (TTC) reductase activity. The succinate-TTC reductase activities of extracts of E. coli grown in the presence of dihydrostreptomycin (resistant and dependent) were relatively low and were not inhibited by the antibiotic. At a moderate magnesium concentration, the degree of inhibition by dihydrostreptomycin of succinate-TTC reductase activity was sufficiently marked to indicate an important site of action of the antibiotic. Magnesium, putrescine and spermidine antagonized the action of dihydrostreptomycin in the succinate-TTC reductase system.