Mass determination of human immunoglobulin IgM using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry
- 1 August 1994
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 8 (8) , 627-631
- https://doi.org/10.1002/rcm.1290080811
Abstract
Monoclonal human immunoglobulin IgM has been analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Using a matrix of sinapinic acid with a laser wavelength of 355 nm, positive ions with 2 to 6 charges were observed in the mass spectra. Two major molecular species were present. The molecular weights estimated from the multiply-charged signals were found to decrease systematically with decrease in the charge state of the ion. This effect was determined to stem from the non-linear influence of the initial kinetic energy of desorption on the final kinetic energy of the multiply-charged species. A method of successive approximations, with the molecular weights and desorption velocities of the analytes as variables, was used to correct for these non-linearities. Mean molecular weights for the two IgM species were found to be 939 000 ± 2000 and 982 000 ± 2000 Da. An initial desorption velocity of 565 ± 10 ms/s was determined for both.This publication has 8 references indexed in Scilit:
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