Cryopreservation of isolated mint shoot tips by vitrification
- 1 August 1990
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 9 (4) , 178-180
- https://doi.org/10.1007/bf00232174
Abstract
Shoot tips isolated from a mint clone, Mentha aquatica x M. spicata, were gradually exposed to a mixture containing 35% ethylene glycol, 1 M dimethylsulfoxide and 10% polyethylene glycol-8000 and then immersed into liquid nitrogen. Cooling and warming rates were approximately 4800°C/min and 9000°C/min respectively. Survival after liquid nitrogen treatment ranged from 31% to 75% among experiments. There was no obvious reason for this variation. In many cases the treated shoot tip directly developed into a shoot without any or with only slight callus formation.This publication has 5 references indexed in Scilit:
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