Autophagocytosis in mouse seminal vesicle cells in vitro

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Abstract
Summary Spontaneous autophagocytosis was observed in mouse seminal vesicle cells during incubation for 2 h in vitro. The number of autophagic vacuoles formed was greatest at 37° C and decreased when the temperature was lowered. At 22° C it reached the near-zero value characteristic of nonincubated control cells. Incubation of the cells at 37° C in the presence of 0.1 mg/ml vinblastine sulfate resulted in a marked increase in the number of autophagic vacuoles, but the drug was ineffective at 22° C. Puromycin (10−3 M) exerted no influence on spontaneous autophagocytosis, but cycloheximide in concentrations from 10−7M to 10−3M inhibited both spontaneous and vinblastine-induced autophagocytosis. The formation of tubulin paracrystals in vinblastine treated cells was not prevented either by low (22° C) temperature or in the presence of cycloheximide.