Studies on the Synthesis of Phosphoglycerides in Escherichia coli

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Abstract
The incorporation of various labelled precursors into E. coli lipids was studied. In growing cells, incorporation of glycerol, doubly labelled with 14C and 3H, occurs without a change in isotope ratio. In cell homogenates incorporation of sn-glycero-3-phosphate-U-14C was greater in the polyglycerophosphatide fraction than in phosphatidylethanolamine and higher in the unacylated than in the acylated glycerol moiety of phosphatidylglycerol. This uneven distribution of label in phosphatidylglycerol was not affected by addition of unlabelled dihydroxyacetone phosphate and occurred to the same extent when glucose-3,4-14C was the precursor. Our results obtained in vivo indicate that glycerophosphate is acylated without prior oxidation. Furthermore, labelling patterns obtained in vitro suggest an extensive dilution by endogenous diacyl glycerol precursors not formed by acylation of dihydroxyacetone phosphate.

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