Inhibition of apoptosis by the expression of antisense Nedd2
- 10 July 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 368 (1) , 69-72
- https://doi.org/10.1016/0014-5793(95)00602-6
Abstract
Nedd2 belongs to a family of mammalian cysteine proteases which share similarity with the Caenorhabditis elegans cell death protein, Ced‐3. Overexpression of Nedd2 has been shown to induce apoptosis in mammalian cells but in the absence of a specific known inhibitor, it remains to be seen whether this represents cytotoxic effects of the Nedd2 protease or the specific activation of an apoptotic pathway. The present work shows that the factor‐dependent cell line FDC‐P1 expressing mouse antisense Nedd2 mRNA, exhibits significant inhibition of cell death upon removal of the cytokines, thus providing evidence for a direct role of Nedd2 in mediating apoptosis.Keywords
This publication has 28 references indexed in Scilit:
- Altered Cytokine Export and Apoptosis in Mice Deficient in Interleukin-1β Converting EnzymeScience, 1995
- Induction of apoptosis in fibroblasts by IL-1β-converting enzyme, a mammalian homolog of the C. elegans cell death gene ced-3Cell, 1993
- The C. elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1β-converting enzymeCell, 1993
- Identification of a set of genes with developmentally down-regulated expression in the mouse brainBiochemical and Biophysical Research Communications, 1992
- Viral inhibition of inflammation: Cowpox virus encodes an inhibitor of the interleukin-1β converting enzymeCell, 1992
- A novel heterodimeric cysteine protease is required for interleukin-1βprocessing in monocytesNature, 1992
- Efficient selection for high-expression transfectants with a novel eukaryotic vectorGene, 1991
- Activation of interleukin‐ 1β by a co‐induced proteaseFEBS Letters, 1989
- Activation and growth of colony-stimulating factor-dependent cell lines is cell cycle stage dependent.The Journal of Experimental Medicine, 1987
- Complete replacement of serum in primary cultures of erythropoietin-dependent red cell precursors (CFU-E) by albumin, transferrin, iron, unsaturated fatty acid, lecithin and cholesterolExperimental Cell Research, 1980