ULTRAVIOLET LIGHT INDUCED LINKING OF DEOXYRIBONUCLEIC ACID STRANDS AND ITS REVERSAL BY PHOTOREACTIVATING ENZYME
- 1 June 1961
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 47 (6) , 778-787
- https://doi.org/10.1073/pnas.47.6.778
Abstract
The irradiation of DNA in solution with UV light with doses approximating those used for inactivation of transforming activity causes (1) the irreversible hydrogen-bond breakage, most likely in internal regions of the DNA molecule and (2) the formation of formamide-and heat-stable linkages between the two complementary DNA strands. Repair of the latter damage is catalyzed by the photoreactivating enzyme from bakers'' yeast The cross-links are in keeping with the observations of others on the irradiation of thymine in the frozen state and may be due to the formation of dimers of thymine.Keywords
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