Hyaluronidase activity in lysosomes of bone tissue

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Abstract
The distribution pattern of hyaluronidase in subcellular fractions of bone-tissue homogenates is closely similar to that reported for the other acid hydrolases of this tissue. The highest specific activity of hyaluronida.se is also found in the light-mitochondrial fraction. In cytoplasmic extracts of bone, about 60 percent of the activity of hyaluronidase is latent, and is unmasked by a number of treatments (digitonin, low osmotic pressure, freezing and thawing, Waring Blendor) that unmask the lysosomal B -glucuronidase than of hyaluronidase accessible to external substrates, but release the same proportion of both enzymes in unsedi-mentable form. These results support the concept of an association of hyaluronidase with lysosomes in bone.