Measurement of glucose and 2‐deoxy‐2‐[18F]fluoro‐D‐glucose transport and phosphorylation rates in myocardium using dual‐tracer kinetic experiments

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Abstract
To examine the use of 2‐deoxy‐2‐[18F]fluoro‐D‐glucose (2‐FDG) as a glucose analog for measuring glucose utilization rate in myocardium, dual‐tracer kinetic experiments with 2‐FDG and 2‐[3H]glucose were performed in the perfused, isolated rabbit interventricular septum to measure simultaneously the transport and phosphorylation rates of glucose and 2‐FDG. Results of the present study indicated that, in the septum, (i) the transport rate constants of 2‐FDG and glucose were similar in magnitude, (ii) the phosphorylation rate constant for 2‐FDG was about 60% of that of glucose, (iii) hypoxia caused an increase in phosphorylation rates of glucose and 2‐FDG without affecting transport.