DNA microarray reveals changes in gene expression of shear stressed human umbilical vein endothelial cells
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- 31 July 2001
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 98 (16) , 8955-8960
- https://doi.org/10.1073/pnas.171259298
Abstract
Using DNA microarray screening (GeneFilter 211, Research Genetics, Huntsville, AL) of mRNA from primary human umbilical vein endothelial cells (HUVEC), we identified 52 genes with significantly altered expression under shear stress [25 dynes/cm2 for 6 or 24 h (1 dyne = 10 μN), compared with matched stationary controls]; including several genes not heretofore recognized to be shear stress responsive. We examined mRNA expression of nine genes by Northern blot analysis, which confirmed the results obtained on DNA microarrays. Thirty-two genes were up-regulated (by more than 2-fold), the most enhanced being cytochromes P450 1A1 and 1B1, zinc finger protein EZF/GKLF, glucocorticoid-induced leucine zipper protein, argininosuccinate synthase, and human prostaglandin transporter. Most dramatically decreased (by more than 2-fold) were connective tissue growth factor, endothelin-1, monocyte chemotactic protein-1, and spermidine/spermine N1-acetyltransferase. The changes observed suggest several potential mechanisms for increased NO production under shear stress in endothelial cells.Keywords
This publication has 40 references indexed in Scilit:
- Differential Response of Cultured Human Umbilical Vein and Artery Endothelial Cells to Ah Receptor Agonist Treatment: CYP-Dependent Activation of Food and Environmental MutagensToxicology and Applied Pharmacology, 2000
- Effects of Mechanical Forces on Signal Transduction and Gene Expression in Endothelial CellsHypertension, 1998
- Predominant role for nitric oxide in the relaxation induced by vasoactive intestinal polypeptide in human uterine arteryMolecular Human Reproduction, 1998
- Cellular and molecular mechanisms of endothelial cell dysfunction.Journal of Clinical Investigation, 1997
- Intracellular Calcium Mobilization Suppresses the TNF-α–Stimulated Synthesis of PAI-1 in Human Endothelial CellsArteriosclerosis, Thrombosis, and Vascular Biology, 1997
- Differential Display and Cloning of Shear Stress-Responsive Messenger RNAs in Human Endothelial CellsBiochemical and Biophysical Research Communications, 1996
- Expression of Cytochrome P450s and Microsomal Epoxide Hydrolase in Primary Cultures of Human Umbilical Vein Endothelial CellsToxicology and Applied Pharmacology, 1994
- A Comparison of Substrates for Human Umbilical Vein Endothelial Cell CultureBiotechnic & Histochemistry, 1992
- Tissue plasminogen activator messenger RNA levels increase in cultured human endothelial cells exposed to laminar shear stressJournal of Cellular Physiology, 1990
- Culture of Human Endothelial Cells Derived from Umbilical Veins. IDENTIFICATION BY MORPHOLOGIC AND IMMUNOLOGIC CRITERIAJournal of Clinical Investigation, 1973