Factors Influencing in Vitro Multiplication and Rooting of the Plum Rootstock Myrobalan (Prunus cerasifera Ehrh.)1

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Abstract
Shoot tips (5 mm) of Myrobalan plum were cultured on Stage I micropropagation medium consisting of liquid modified Murashige and Skoog salts (MS) medium supplemented with 2% sucrose and in mg/liter: 0.4 thiamine HCl, 100 myo-inositol, 0.5 pyridoxine * HCl, 0.5 nicotinic acid, 0.1 p-aminobenzoic acid, 0.01 indolebutyric acid (IBA) and 0.26-benzylamino purine (BA). A 10-fold multiplication rate was achieved every 4–6 weeks when cultured shoots were transferred to Stage II multiplication medium consisting of Stage I medium with 1.0 mg/liter BA and 0.6% agar. Complete rooting (100%) occurred at 4 weeks when in vitro-proliferated shoots were transferred to MS medium supplemented with 2.5 – 5.0 mg/liter indoleacetic acid (IAA) and incubated in the dark for 2 weeks at either 21° or 26°C. Poor rooting occurred in the light (1.0 klx, 16-hr photoperiod). Shoots incubated at 26°C rooted more quickly than those at 21°C. Shoots cultured on MS medium with IAA and incubated in the dark for 2 weeks rooted significantly better than shoots on medium with IBA and incubated under dark or light conditions. Addition of chlorogenic acid to the rooting medium significantly increased rooting in the light. Plantlets were successfully transferred to soil.

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