Treatment of HL-60 cells with dimethyl sulphoxide inhibits the formation of β-N-acetylhexosaminidase S
- 15 November 1990
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 272 (1) , 211-215
- https://doi.org/10.1042/bj2720211
Abstract
.beta.-N-Acetylhexosaminidase of HL-60 cells was separated into two main forms, A and S, by chromatography on DEAE-cellulose. Analysis of developmental changes in the isoenzyme pattern was complicated by the fact that the specific activity of .beta.-N-acetylhexosaminidase underwent a 6-fold change during the normal growth cycle. Two other lysosomal enzymes, .beta.-galactosidase and .alpha.-mannosidase, behaved similarly. Induction of differentiation of HL-60 cells with dimethyl sulphoxide at a low cell density (3 .times. 105 cells/ml) had a greater effect on the abundance of .alpha.-subunits of .beta.-N-acetylhexosaminidase, measured with 4-methylumbelliferyl-.beta.-N-acetylglucosaminide 6-sulphate, than of .beta.-subunits, measured with 4-methylumbelliferyl-.beta.-N-acetylglucosamine, and resulted in an isoenzyme profile in which A and B were the major forms, with the levels of form S greatly decreased.This publication has 23 references indexed in Scilit:
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