Separation of the Main Neutral Lipids into Classes and Species by PR-HPLC and UV Detection

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Abstract
A reversed phase high performance liquid chromatographic method is described that allows separation and estimation of the main neutral lipid classes as well as several species of each class. By the represented method waxes, hydrocarbons, fatty acids and their methyl esters, sterols and their esters, free glycerylethers, fatty alcohols, vitamin E and mono-, di- and triglycerides are separated into classes and into class species within 55min. A stepped gradient elution with methanol/water, acetonitrile/methanol, acetonitrile/tetrahydrofuran and isopropanol/acetonitrile was performed onto a reverse phase C18 HPLC column and the effluent was monitored by UV detection at 206 nm. Application of the method in a plant extract and in a vegetable oil sample is represented.