Serologic Diagnosis of Lyme Disease using Recombinant Outer Surface Proteins A and Band Flagellin

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Abstract
Recombinant (r) outer surface proteins (Osp) A and B, flagellin, and an immunogenicregion of flagellin (41-G) from Borrelia burgdorferi were evaluated using immunoblot and ELISA for usefulnessas substrates in diagnostic testing for Lyme disease. Antibodiesto rOspA, rOspB, and r-flagellin were detected by immunoblot and ELISA using the recombinant proteins. Patients with late disease responded to rOspA, rOspB, and r-flagellin, or only to r-flagellin, whereas patients with early disease showed no response or responded only to r-flagellin, Patients who developed antibodies to r-flagellin also responded to 41-G. The data suggest that recombinant B. burgdorferi antigens can serveas substrates for immunoblot and ELISA, which maybe helpful in diagnosing Lyme disease.

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