Comparison of Various Procedures to Detect Neutralizing Antibody to the Channel Catfish Virus in California Brood Channel Catfish
- 1 January 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in The Progressive Fish-Culturist
- Vol. 46 (1) , 6-12
- https://doi.org/10.1577/1548-8640(1984)46<6:covptd>2.0.co;2
Abstract
The first outbreak of channel catfish virus disease (CCVD) since 1973 occurred in California in the fall of 1981 in channel catfish (Ictalurus punctatus). In order to determine the possible spread of CCV to other farms, brood catfish were tested for serum neutralizing (SN) antibody to CCV. Procedures that are commonly used to detect SN antibody in catfish sera gave conflicting results, and tests were conducted to resolve the discrepancy. The lack of standardization caused the variance in results because the brown bullhead (BB) cell line was significantly less (P > .05) sensitive to CCV than the channel catfish ovary (CCO) cell line, less virus could be detected in preformed CCO cell monolayers as compared with the drop technique, and the ability to detect SN was affected by the dilution of serum. The procedure adopted for detecting SN antibody to CCV was to mix equal volumes of CCV solution containing 100 infective doses-50% with sera diluted 1:25 for 1 hr at 25 C, then inoculate BB cells into 6 ...Keywords
This publication has 1 reference indexed in Scilit:
- CHANNEL CATFISH VIRUS: COMPARATIVE REPLICATION AND SENSITIVITY OF CELL LINES FROM CHANNEL CATFISH OVARY AND THE BROWN BULLHEADJournal of Wildlife Diseases, 1980