ANTITUMOR ACTIONS OF KEOXIFENE AND TAMOXIFEN IN THE N-NITROSOMETHYLUREA-INDUCED RAT MAMMARY-CARCINOMA MODEL

  • 1 August 1987
    • journal article
    • research article
    • Vol. 47  (15) , 4020-4024
Abstract
We have compared the antitumor activities of the antiestrogens, keoxifene (LY 156758) and tamoxifen (TAM), using the N-nitrosomethylurea (NMU) rat mammary carcinoma model. To established an effective antitumor dose of TAM in this model, rats were treated 2 wk after initiation with NMU for 8 wk with s.c. daily injections of 6.25 .mu.g, 25 .mu.g, or 100 .mu.g of TAM in peanut oil. At the 25-.mu.g and 100-.mu.g daily doses, TAM completely inhibited tumor appearance during the therapy period. An effective antitumor dose of TAM (100 .mu.g daily) was compared to 20-, 100-, or 500-.mu.g daily doses of keoxifene 7 wk after NMU initiation. None of the keoxifene-treated groups prevented the appearance of tumors as effectively as TAM during 13 wk of therapy. When keoxifene was compared to TAM at equivalent daily doses of 100 and 500 .mu.g daily starting 2 wk after NMU injection, the keoxifene groups again failed to prevent the appearance of all tumors during 10 wk of therapy. TAM, however, completely suppressed any tumor formation. In the same experiment, animals treated with 500 .mu.g of TAM had therapy stopped after 10 wk, and tumors started to appear 6 wk later. No tumors appeared when animals (n = 25) were treated continuously for 23 wk with 100 .mu.g of TAM. In separate experiments, keoxifene (500 .mu.g daily) and TAM (500 .mu.g and 100 .mu.g daily) administered for 1 wk blocked the binding of [3H]-estradiol in NMU tumors and in uteri. The effect lasted for up to 5 wk after antiestrogen therapy was stopped. These experiments demonstrate that keoxifene is not as effective in its antitumor action as TAM in the NMU model.