Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides

Open Access

1 January 1985

journal article
Published by Oxford University Press (OUP) in Nucleic Acids Research

Vol. 13 (1) , 45-57
https://doi.org/10.1093/nar/13.1.45

Abstract

Biotin has been converted to 2-(biotinylamido)ethanol and condensed to phosphorylated oligonucleotides in a solid phase synthesis. The 5′-biotinylated oligonucleotides were enzymatically coupled to other DNA fragments by T4 DNA ligase or T4 RNA ligase. The hybridization properties of such biotin-labeled oligonucleotide probes were studied.

Keywords

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