Effects of low temperatures on survival of frozen-thawed mouse embryos
- 1 November 1979
- journal article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 35 (11) , 1505-1506
- https://doi.org/10.1007/bf01962812
Abstract
Almost no damage to mouse morulae was observed between 0 and −40°C, and freezing damage to embryos in DMSO, glycerol or ethylene glycol occurred after exposure to −60, −50 or −50°C, respectively. Cooling embryos in DMSO or glycerol to temperatures below −50 to −60°C increased freezing damage. To the contrary, in the presence of ethylene, glycol, no more damage occurred after exposure to temperatures below −50°C.Keywords
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