An Improved Extender for Freezing Atlantic Salmon Spermatozoa

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Abstract
A solution of chemical composition based on that of seminal plasma was an effective extender for Atlantic salmon spermatozoa; semen diluted to 20 volumes could be maintained at 4 C in an inactive but potentially motile condition for several days. Propylene glycol at concentrations between 7.0 and 12.5% protected sperm cells against freeze–thaw damage. The addition of glycine or albumin to the basic extender containing 7–12.5% propylene glycol or 10% dimethyl sulphoxide provided an extender meriting further development for the cryopreservation of salmonid spermatozoa. Fertilities of 5–19% were achieved with 10 frozen sperm samples diluted with such extenders. For frozen samples a method of fertilization with simultaneous addition of water and sperm to the eggs is proposed.

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