RNA synthesis in preovulatory mouse oocytes.

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Abstract
RNA synthesis, previously shown to take place during oocyte growth, was demonstrated throughout the growth-quiescent period preceding ovulation of the mouse oocyte. In the final 7-day preovulatory period the level of incorporation of [5,6-3H]uridine into ovulated oocytes decreased as the interval between exposure to precursor and ovulation decreased; significant incorporation was detectable within 2 days before ovulation. Analysis of the frequency and density of label in ovarian oocytes at successive stages of meiosis in relation to the interval between administration of labeled precursor and collection of oocytes revealed that RNA synthesis continues up to within 2 h before germinal vesicle breakdown.