Efficient transformation of Lactococcus lactis IL1403 and generation of knock‐out mutants by homologous recombination
- 23 May 2007
- journal article
- research article
- Published by Wiley in Journal of Basic Microbiology
- Vol. 47 (3) , 281-286
- https://doi.org/10.1002/jobm.200610297
Abstract
Lactococcus lactis IL1403 is a Gram‐positive bacterium of great biotechnological interest for food grade applications. Its use is however hampered by the difficulty to efficiently transform this strain. We here describe a detailed, optimized electrotransformation protocol which yields a transformation efficiency of 106 cfu/μg of DNA with the two E. coli Gram‐positive shuttle vectors pC3 and pVA838. The utility of the protocol was demonstrated by the generation of single‐ and double‐knock‐out mutants by homologous recombination.Keywords
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