Inhibition of Proline-C14 Incorporation into Rat Liver Ribosomes by Thiazolidine-4-Carboxylic Acid in a Cell-Free System.

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Abstract
Summary Thiazolidine-4-carboxylic acid (TZCA) was used to inhibit the incorporation of proline into rat liver ribosomes in a cell-free system. It was found that TZCA was incorporated into ribosomes via a pathway similar to that of proline. The coding polynucleotide for this incorporation was a homo-polymer of cytidine. t-RNA-TZCA-S35 was also isolated, and this presented further evidence that TZCA-S35 was incorporated into ribosomes following the formation of t-RNA-TZCA-S35. Hence, these experiments show that TZCA may be used as a competitive inhibitor to the incorporation of proline into ribosomes in a cell-free system.