IDENTIFICATION OF HB D-PUNJAB GENE - APPLICATION OF DNA AMPLIFICATION IN THE STUDY OF ABNORMAL-HEMOGLOBINS

  • 1 June 1989
    • journal article
    • research article
    • Vol. 44  (6) , 886-889
Abstract
Hemoglobin D-Punjab (or D-Los Angeles) in a common variant worldwide. It is also the most frequent abnormal hemoglobin in Xinjiang Uygur Autonomous Region of China. A large survey of hemoglobinopathy, including 142,171 people and 21 national/ethnic groups, was carried out in Xinjiang and indicated Hb D-Punjab accounted for 55.6% of the total hemoglobin variants there. Here we descirbe a simple way-EcoRI mapping of the amplified .beta.-globin DNA sampling from dried blood spots on filter paper blotters-of identifying the Hb D-Punjab gene. The primers were designed and synthesized to emzymatically amplify a 144-bp fragment of .beta.-globin gene which included codons .beta. 121 (GAA) and 122 (TTC) representing an EcoRI recognition site. The Hb D-Punjab gene could be easily detected by EcoRI digestion of the amplified DNA sequence of agarose gel because of a single base change at codon 121. The analysis of amplified DNA sampling from dried blood provides a very useful method for population study of Hb D-Punjab and will be of significance for demonstration of the occurrence of the Hb D-Punjab gene and for understanding of the relations among various nationalities.