Endogenous indoles and the biosynthesis and metabolism of indole-3-acetic acid in cultures of Rhizobium phaseoli
- 1 July 1987
- journal article
- research article
- Published by Springer Nature in Planta
- Vol. 171 (3) , 422-428
- https://doi.org/10.1007/bf00398689
Abstract
Gas chromatography-mass spectrometric analyses of purified extracts from cultures of Rhizobium phaseoli wild-type strain 8002, grown in a non-tryptophan-supplemented liquid medium, demonstrated the presence of indole-3-acetic acid (IAA), indole-3-ethanol (IEt), indole-3-aldehyde and indole-3-methanol (IM). In metabolism studies with 3H-, 14C- and 2H-labelled substrates the bacterium was shown to convert tryptophan to IEt, IAA and IM; IEt to IAA and IM; and IAA to IM. Indole-3-acetamide (IAAm) could not be detected as either an endogenous constituent or a metabolite of [3H]tryptophan nor did cultures convert [14C]IAAm to IAA. Biosynthesis of IAA in R. phaseoli, thus, involves a different pathway from that operating in Pseudomonas savastanio and Agrobacterium tumefaciens-induced crown-gall tumours.Keywords
This publication has 16 references indexed in Scilit:
- Genes Specifying Auxin and Cytokinin Biosynthesis in PhytopathogensAnnual Review of Plant Physiology, 1986
- Biosynthetic and Metabolic Aspects of AuxinsPublished by Springer Nature ,1986
- Catabolism of indole‐3‐acetic acid to indole‐3‐methanol in a crude enzyme extract and in protoplasts from Scots pine (Pinus sylvestris)Physiologia Plantarum, 1985
- Phytohormones,Rhizobium mutants, and nodulation in legumes. IV. Auxin metabolites in pea root nodulesJournal of Plant Growth Regulation, 1984
- The biosynthesis of indole-3-acetic acid byFrankiaPlant and Soil, 1984
- Mass spectrometric identification of indole compounds produced byRhizobium strainsJournal of Mass Spectrometry, 1982
- Growth regulators, Rhizobium and nodulation in peasPlanta, 1982
- Preparation of radioactively labelled indole‐3‐acetic acid precursorsJournal of Labelled Compounds and Radiopharmaceuticals, 1981
- Factors responsible for the curling and branching of clover root hairs by RhizobiumPlant and Soil, 1976
- Ammonia Assimilation by Rhizobium Cultures and BacteroidsJournal of General Microbiology, 1975