Estimation of bovine colostral immunoglobulins by refractometry
- 12 July 1980
- journal article
- research article
- Published by Wiley in Veterinary Record
- Vol. 107 (2) , 35-36
- https://doi.org/10.1136/vr.107.2.35
Abstract
The total protein of colostral whey from dairy cows as determined by a refractometer were compared with the immune globulin concentration obtained by cellulose acetate membrane electrophoresis and the immunoglobulin (IgA + IgG + IgM) contents determined by radial immunodiffusion. The coefficient of correlation between the results obtained by refractometry and electrophoresis was 0.98 (P < 0.001). The correlation between refractometry and radial immunodiffusion was 0.89 (P < 0.001), while that of electrophoresis and radial immunodiffusion was 0.87 (P < 0.001). Refractometry can be used as a simple, fast and inexpensive indirect method of assessing immunoglobulins in colostral whey.Keywords
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