Tissue‐specific expression of cre recombinase from the Pax8 locus
- 26 February 2004
- Vol. 38 (3) , 105-109
- https://doi.org/10.1002/gene.20008
Abstract
The transcription factor Pax8 is expressed in the developing thyroid gland, inner ear, kidney, and mid-hindbrain region. Pax8 mutant mice die only postnatally due to a thyroid gland defect. Here we report the generation and expression analysis of a Pax8cre allele. Cre recombinase activity was detected in known Pax8 expression domains of Pax8cre/+ and Pax8cre/cre embryos carrying the Z/AP transgene, which expresses alkaline phosphatase only after Cre-mediated excision of lacZ sequences. Alkaline phosphatase expression was additionally detected in the adrenal gland and in the facial, vestibulocochlear, and cuneate nerves, which have so far not been associated with Pax8 expression. Our data indicate that the Pax8cre allele provides a novel important tool for conditional gene manipulation and lineage tracing in different Pax8 expression domains. genesis 38:105–109, 2004.Keywords
This publication has 10 references indexed in Scilit:
- Nephric lineage specification by Pax2 and Pax8Genes & Development, 2002
- Conditional control of gene expression in the mouseNature Reviews Genetics, 2001
- Pax5/BSAP Maintains the Identity of B Cells in Late B LymphopoiesisImmunity, 2001
- Z/EG, a double reporter mouse line that expresses enhanced green fluorescent protein upon cre-mediated excisionGenesis, 2000
- High-efficiency deleter mice show that FLPe is an alternative to Cre-loxPNature Genetics, 2000
- Z/AP, a Double Reporter for Cre-Mediated RecombinationDevelopmental Biology, 1999
- Characterization of three novel members of the zebrafish Pax2/5/8 family: dependency of Pax5 and Pax8 expression on the Pax2.1 (noi) functionDevelopment, 1998
- Follicular cells of the thyroid gland require Pax8 gene functionNature Genetics, 1998
- Pax8, a murine paired box gene expressed in the developing excretory system and thyroid glandDevelopment, 1990
- Organogenesis of the KidneyPublished by Cambridge University Press (CUP) ,1987