Some Properties of the DCMU-Binding Site in Chloroplasts
Open Access
- 1 November 1979
- journal article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 34 (11) , 992-995
- https://doi.org/10.1515/znc-1979-1122
Abstract
Binding of the triazinone derivative metribuzin to thylakoid membranes was studied in dependence of temperature and trypsin pre-treatment of the chloroplasts. The temperature curve of the binding constant suggests that the lipid phase of the membrane contributes to inhibitor binding. Trypsin pre-treatment of the membranes removes the receptor sites for the inhibitor, while the trypsin effect on the affinity of the receptor to the inhibitor is marginal. Concomitantly trypsin treatment releases the inhibitory effect of metribuzin on the electron transport from DPC to DCPIP.Keywords
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