Dynamic Monitoring of Organic Cation Transport Processes by Fluorescence Measurements in LLC-PK1 Cells
- 1 January 1996
- journal article
- research article
- Published by S. Karger AG in Cellular Physiology and Biochemistry
- Vol. 6 (1-2) , 72-81
- https://doi.org/10.1159/000154796
Abstract
The permanent cationic fluorescent probe 4-(4-dimethylaminostyryl)-N-methylpyridinium (ASP+) has recently been reported to be transported by the organic cation transporters in the proximal tubule of the kidney [Kidney Int 1995;47:1647-1657]. A new application for the fluorescence microscopy technique using this dye as a substrate for organic cation transport systems is described, which can be used for dynamic monitoring of organic cation transport in cultured renal cells of proximal origin (LLC-PK1 cells). The uptake of ASP+ was concentration dependent and linear over the first 10 min at a concentration of 1 µmol/l. The results are compatible with a carrier-mediated transport process presenting characteristics of organic cation transport systems.Keywords
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