Oligodeoxyribonucleotides: Chemical Synthesis in Anhydrous Base
- 27 February 1970
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 167 (3922) , 1266-1268
- https://doi.org/10.1126/science.167.3922.1266
Abstract
The sugar hydroxyl of a methoxytrityldeoxyribonucleoside reacts with a methoxytrityldeoxyribonucleoside phosphorofluoridate in dimethylformamide and potassium tertiary butoxide to yield the protected dinucleoside monophosphate. The reaction is fast and specific, and is used in a stepwise synthesis to prepare trinucleoside diphosphate and tetranucleoside triphosphate.Keywords
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