The analysis of mitoses in single living cells by interference microscopy

Abstract
A new method of using the interference microscope is described which enables a continuous record to be obtained of changes in dry mass and concentration during the growth of a single tissue cell. The method has been applied to the analysis of changes of dry mass in the chromosomes, spindle and cell-plate area during mitosis in endosperm. The results of these observations have been compared with earlier studies of birefringence during mitosis. It is shown that birefringence develops under conditions of progressively increasing concentration of macro-molecular material.

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