Isolation ofCeratocystis wagenerifrom Forest Soil with a Selective Medium

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Abstract
A selective medium containing 800 .mu.g/ml cycloheximide was developed for the isolation of C. wageneri from soil. Fifty-nine soil samples were collected from around roots of infected ponderosa pines [Pinus ponderosa] and assayed. In 11 cases, C. wageneri was isolated in quantities of up to 12,000 propagules/g of soil and, in one case, was found 4-6 cm from the infected root. Most propagules apparently were conidia but several bits of mycelium removed from soil also proved to be C. wageneri. Mycelial growth through soil for short distances may be an important means of spread of the pathogen. Other species of Ceratocystis were isolated from soil and were tolerant of high concentrations of cycloheximide.

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