Effect of free-cell growth parameters on oxygen concentration profiles in gel-immobilized recombinantEscherichia coli
- 1 September 1990
- journal article
- research article
- Published by Springer Nature in Applied Microbiology and Biotechnology
- Vol. 33 (6) , 619-623
- https://doi.org/10.1007/bf00604925
Abstract
Oxygen concentration profiles in immobilized recombinant cells were measured using a microsensor. Experimental results showed that the final depth of oxygen penetration in the carrageenan gel was always in the range 80–100 μm for the strains and media used, although profiles of oxygen concentration during the early stages of cell growth depended on the strain and nutrient medium used. Variations in the oxygen profiles corresponded to differences in kinetic parameters measured for the same strains and media in free-cell experiments.Keywords
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