Secretory cleavage site of Alzheimer amyloid precursor protein is heterogeneous in Down's syndrome brain
- 5 September 1994
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 351 (2) , 165-167
- https://doi.org/10.1016/0014-5793(94)00851-5
Abstract
Aβ (β/A4) is the major constituent of brain amyloid in Alzheimer's disease (AD), Down's syndrome (DS) and normal aged persons. This protein is presumably derived by normal proteolysis from a precursor protein (APP). In this study, C-terminal fragments of APP in a Tris/Triton soluble fraction were partially purified from DS brain by heparin-affinity and reverse phase chromatography, and analyzed by N-terminal amino acid sequencing after SDS polyacrylamide gel electrophoresis and Western blotting. We found at least six different C-terminal fragments including those with the entire Aβ region. These results suggest that secretory processing of APP is heterogeneous and generates amyloidogenic C-terminal fragmentsKeywords
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