Interaction of wild-type and variant mouse 3T3 cells with lectins from Bandeiraea simplicifolia seeds.

Publisher Website
Google Scholar
Abstract
An isolectin (BSI-B4) derived from B. simplicifolia seeds and specific for terminal .alpha.-D-galactopyranosyl groups was cytotoxic to Swiss 3T3 mouse [fibroblast] cells. After mutagenesis and selection with BS I-B4, a variant clonal cell line resistant to both this isolectin and the .alpha.-D- and .beta.-D-galactose-binding lectin abrin was isolated. The parental cell line showed homogeneous and noninteracting binding sites for BS I-B4, whereas the variant cells exhibited a curved plot with a reduced number of binding regions. Another lectin, BS II, which is derived from the same seeds, but specific for terminal N-acetyl-D-glucosaminyl groups, was cytotoxic to the variant but not the parental cells. A possible lesion in the biosynthesis of cell surface structures resulting in the exposure of subterminal N-acetyl-D-glucosaminyl moieties in the variant line is suggested.