An Improved Thaw‐Siphon Method for the Cryoprecipitate Preparation

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Abstract
Cryoprecipitates were prepared by various techniques including slow‐thaw, rapid‐thaw, thaw‐centrifuge and thaw‐siphon methods. Recoveries of 42.3 ±9.5% for slow‐thaw, 48.8 ±8.8% for rapid‐thaw, 41.3 ±15.8% for thaw‐centrifuge and 67.4 ±8.9% for thaw‐siphon in factor VIII activity were obtained. These results indicated that cryoprecipitate prepared by the thaw‐siphon method had the best recovery of the factor VIII procoagulant activity. However, the final volume of the plasma was rather difficult to control and there was a risk that some factor‐VIII‐containing materials were siphoned with the cryo‐poor plasma, especially approaching the end of the process. A modified thaw‐siphon method was developed which involved stopping the siphon after 60 min, centrifuging and expressing the remaining plasma to a final volume of less than 15 ml. A yield of 67.1 ±9.8% factor VIII activity was obtained. This product is similar to that produced by the original thaw‐siphon method in yield but about half in volume.