Cloning of the Vibrio harveyi luciferase genes: use of a synthetic oligonucleotide probe.
Open Access
- 1 January 1983
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 80 (1) , 120-123
- https://doi.org/10.1073/pnas.80.1.120
Abstract
A mixed-sequence synthetic oligonucleotide probe was used to isolate a clone containing the gene encoding the .alpha. subunit of bacterial luciferase from V. harveyi [Beneckea harveyi] and part of the gene coding for the .beta. subunit. DNA sequence analysis revealed that the genes are closely linked on the bacterial chromosome and transcribed in the same direction. Comparison of the sequences in the regions preceding the 2 structural genes revealed considerable homology and identified sites that may be involved in the expression of the genes. Identification of a clone from a clone bank of total genomic DNA from this organism shows that mixed probes can be successfully used to isolate a gene of interest from any bacterium provided some protein sequence for the gene product is available.This publication has 24 references indexed in Scilit:
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