Induction of Differentiation in the Shoot Apical Meristem by Transient Overexpression of a Retinoblastoma-Related Protein
- 30 June 2006
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 141 (4) , 1338-1348
- https://doi.org/10.1104/pp.106.083022
Abstract
The shoot apical meristem contains cells that undergo continual growth and division to generate the building blocks for the aerial portion of the plant. As cells leave the meristem, they undergo differentiation to form specific cell types. Most notably, heterotrophic cells of the meristem rapidly gain autotrophic capability by synthesis and assembly of components of the chloroplast. At the same time, cells undergo enlargement via vacuolation. Despite significant advances in the characterization of transcriptional networks involved in meristem maintenance and leaf determination, our understanding of the actual mechanism of meristem cell differentiation remains very limited. Using a microinduction technique, we show that local, transient overexpression of a retinoblastoma-related (RBR) protein in the shoot apical meristem is sufficient to trigger cells in the meristem to undergo the initial stages of differentiation. Taken together with recent data showing that RBR protein plays a key role in restricting stem cell differentiation in the root apical meristem, our data contribute to an emerging picture of RBR proteins as a central part of the mechanism controlling meristem cell differentiation.Keywords
This publication has 50 references indexed in Scilit:
- Control of Developmental Regulators by Polycomb in Human Embryonic Stem CellsCell, 2006
- Formation of primordia and phyllotaxyCurrent Opinion in Plant Biology, 2005
- Plant retinoblastoma homologues control nuclear proliferation in the female gametophyteNature, 2004
- Tumour suppression: Putting on the brakesNature, 2004
- Cell cycle-dependent and cell cycle-independent control of transcription by theDrosophilaE2F/RB pathwayGenes & Development, 2003
- Rb-Mediated Heterochromatin Formation and Silencing of E2F Target Genes during Cellular SenescenceCell, 2003
- Analysis of the Competence to Respond to KNOTTED1 Activity in Arabidopsis Leaves Using a Steroid Induction SystemPlant Physiology, 2003
- Relative expression software tool (REST(C)) for group-wise comparison and statistical analysis of relative expression results in real-time PCRNucleic Acids Research, 2002
- Stringent repression and homogeneous de‐repression by tetracycline of a modified CaMV 35S promoter in intact transgenic tobacco plantsThe Plant Journal, 1992
- Stringent repression and homogeneous de-repression by tetracycline of a modified CaMV 35S promoter in intact transgenic tobacco plantsThe Plant Journal, 1992