C-Terminal Peptidyl-L-Proline Hydrolase Activity of Aspergillus Acid Carboxypeptidase1
- 1 June 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 81 (6) , 1733-1737
- https://doi.org/10.1093/oxfordjournals.jbchem.a131633
Abstract
Aspergillus saitoi acid carboxypeptidase hydrolyzed C-terminal peptidyl-L-proline bonds and released the C-terminal proline from Z-Gly-Pro-Leu-GIy-Pro and Z-Gly-Pro at pH 3.3. Proline liberated by the enzymic reaction was measured by a sensitive colorimetric ninhydrin method in glacial acetic acid at 513 nm. A Km value of 1.0 mM and a kcat value of 009 s−1 for Z-Gly-Pro-Leu-Gly-Pro hydrolysis, and a Km value of 5.0 mM and a kcat value of 0.0045 s−1 for Z-Gly Pro hydrolysis were calculated from Lineweaver-Burk plots.This publication has 0 references indexed in Scilit: