Human T cell recognition of cloned HL A class I gene products expressed on DNA transfectants of mouse mastocytoma P815

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Abstract
Cloned genes for human major histocompatibility complex (MHC) class I antigens were introduced by DNA‐mediated gene transfer into a high‐efficiency transfection recipient (HTR) cell line previously derived from mouse mastocytoma P815. Cell surface expression of HLA‐A3, AW24 and CW3 gene products on P815 transfectants was demonstrated by radioimmune assay and by flow cytometry. The human MHC class I gene products were apparently expressed on P815 transfectants in a form recognized by human cytolytic T lymphocytes (CTL). Human CTL generated in unidirectional mixed lymphocyte culture against AW24+ donor lymphocytes clearly lysed P815‐HLA‐AW24+ transfectant target cells, but not untransfected P815(HTR) controls. Moreover, P815‐HLA transfectants could stimulate in vitro a significant alloreactive human CTL response. Lysis of P815‐HLA transfectant target cells by human CTL was inhibited by a monoclonal antibody directed against human MHC class I gene products. These mouse cell transfectants may be useful for the study of human T cell responses.

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