NEW INVITRO CELL LINE ESTABLISHED FROM HUMAN OAT CELL-CARCINOMA OF LUNG

Google Scholar
  • 1 January 1977
    • journal article
    • research article
    • Vol. 37  (9) , 3088-3095
Abstract
A new tissue culture cell line, OAT-1975, was established from explant culture of human oat cell carcinoma of the lung, characterized and maintained for more than 1 yr. The cells grew in a monolayered sheet with 24 h of population-doubling time and are capable of clonal growth with about 60% plating efficiency. The cells still retain unique morphological and structural characteristics of oat cell carcinoma and showed tumor takes when transplanted into nude mice and conditioned young Syrian Golden hamsters. The chromosome constitution of the cells is hypertriploid with a modal peak at 74. The permanent growth integrity of the present cell line made it possible to utilize it for cell biological studies. Cell inactivation experiments indicated that the cell are sensitive to X-rays and Mitomycin C when compared to the responses of HeLA S3 cells.