PREPARATION OF PROTEIN CONJUGATES WITH ALKOXYPOLYETHYLENE GLYCOLS

Publisher Website
Google Scholar
Abstract
Alkoxypolyethylene glycols can be converted to O-alkoxypolyethyleneglycoxy-S-carboxamidomethyldithiocarbonates. These dithiocarbonates are useful for the preparation of protein conjugates with alkoxypolyethylene glycols. Studies with model compounds suggest that sites of reaction are primarily the amino groups of proteins and, to a lesser extent, the aliphatic and phenolic hydroxyl groups. Antigen E, which is an allergenic protein from ragweed pollen, was modified with different alkoxypolyethylene glycols by this procedure. The size and the hydrophobicity of alkoxypolyethylene glycols used influenced the reduction in antigenic activity of conjugates. [Modified antigens may be of use in human immunotherapy].