Antigenic Analysis of Human Heart Tissue. Antigens with Restricted Organ Distribution in Acid Extracts of Human Myocardium
Open Access
- 1 August 1970
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 105 (2) , 416-425
- https://doi.org/10.4049/jimmunol.105.2.416
Abstract
Antigens of human myocardium solbuilized by acid extraction at pH 2.5 were submitted to further analysis by double diffusion and immunoelectrophoresis using rabbit antisera to fractions of human heart acid extract obtained by DEAE-cellulose chromatography. Combined immunodiffusion and specific absorption tests demonstrated at least six antigens common to cardiac and skeletal muscle in acid extracts of myocardium. An additional heart-specific antigen was demonstrated in such acid extracts as well as in saline washes of myocardium. Of the antigens common to cardiac and skeletal muscle, four were found present in the different mammalian species tested, and two showed specificity for primates. The heart-specific antigen exhibited both species-specific and species-cross-reactive antigenic properties. Rabbit antisera to human heart fractions were reactive also with rabbit antigen common to heart and skeletal muscle and with rabbit heart-specific antigen. The antigens common to cardiac and skeletal muscle varied in susceptibility to proteolytic digestion by Pronase, trypsin and pepsin. The heart-specific antigen was susceptible to all three enzymes.Keywords
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