Quantitative Determination of the α-Amylase Inhibitor fromPhaseolus Vulgarisusing size Exclusion High-Performance Liquid Chromatography

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Abstract
A quantitative determination of the .alpha.-amylase inhibitor from kidney beans, a glycoprotein (MW 49,000), by the use of a TSK-SW 3000 column was described. A correlation coefficient of 0.986 was obtained when the HPLC [high performance liquid chromatography] method was compared with a biological assay using Beckman Enzymatic Amylase-DS Reagent Kit. The within-day and between-day coefficients of variation were 4.8-8.9% and 3.7-11.6%, respectively. This method is reliable and has advantages over the biological assays presently available. [The use of this compound as a starch blocker in humans was described.].