Direct fluorescent antibody technique for differentiation between .ALPHA.- and .BETA.-hemolytic Streptococcus spp.

1 January 1987

journal article
research article
Published by The Japanese Society of Fish Pathology in Fish Pathology

Vol. 22 (2) , 77-82
https://doi.org/10.3147/jsfp.22.77

Abstract

Direct fluorescent antibody technique (FAT) was evaluated as a differentiation method between .alpha.-hemolytic Streptococcus sp. (Kusuda et al., 1976; Kusuda et al., 1978) and .beta.-hemolytic Streptococcus sp. (Minami et al., 1979; Kitao et al., 1981; Ohnishi and Jo, 1981; Ugajin, 1981; Nakatsugawa, 1983) in this report. The staining titer of fluorescent antibodies were examined by using heat-fixed smears of .alpha.- and .beta.-hemolytic Streptococcus sp., S. iniae, S. equisimilis and fish pathogens other than Streptococcus. The fluorescent antibody against .alpha.-hemolytic Streptococcus sp. had a titer of 1:10,000 and reacted only to .alpha.-hemolytic Streptococcus sp. The fluorescent antibody against .beta.-hemolytic Streptococcus sp. had a titer of 1:1,000 and reacted only to .beta.-hemolytic Streptococcus sp. and S. iniae. The data presented here suggest that direct FAT can be utilized for differentiation of .alpha.- and .beta.-hemolytic Streptococcus spp., and that .beta.-hemolytic Streptococcus sp. has a common antigen with S. iniae.

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