Pseudosecretion of Escherichia coli chloramphenicol acetyltransferase by Bacillus subtilis

1 July 1987

journal article
research article
Published by American Society for Microbiology in Journal of Bacteriology

Vol. 169 (7) , 3362-3364
https://doi.org/10.1128/jb.169.7.3362-3364.1987

Abstract

Bacillus subtilis harboring the vector 25RBSII secrets an Escherichia coli-derived chloramphenicol acetyltransferase into culture supernatants. The secreted enzyme lacks 18 amino acids; these are removed externally rather than during secretion.

Keywords

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