Linkage of beta 2-microglobulin and ly-m11 by molecular cloning and DNA-mediated gene transfer.

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Abstract
.beta.2-Microglobulin (.beta.2m) is expressed on the cell surface after introduction of a .beta.2mb (C57BL/6N) genomic clone into thymidine kinase-deficient mouse L cells by cotransformation using the Ca(PO4)2 precipitate method. Stable transformant cell lines were identified that express the .beta.2mb allele, as determined by reaction of the cells with appropriate monoclonal antibodies and by 2 dimensional gel electrophoresis of endogenously labeled immunoprecipitates of cell extracts. These .beta.2mb transformants now express ly-m11.2, as detected by an indirect radioimmunoassay. A plasmid subclone of the .beta.2mb gene that contains an 8.4-kilobase insert, after introduction into mouse L cells, similarly directs the synthesis of both the .beta.2mb and the ly-m11.2 antigens. Thus, the .beta.2mb and ly-m11.2 determinants most likely represent sites on the same protein structure.