Biosynthesis of vitamin B12: use of a single 13C label in the macrocycle to confirm C-11 methylation in precorrin-6x

Abstract
[11-13C]Uro'gen III is unambiguously synthesised for enzymic conversion into precorrin-6x which as its octamethyl ester gives a 13C NMR spectrum confirming the presence of a C-11 methyl group.

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